![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K2070016
Designed by: iGEM UT-Tokyo 2016 Group: iGEM16_UT-Tokyo (2016-10-09)
Constitutive ecf16 generator
This part contains an ECF16 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101). The ecf16 generated can activate the ecf16 promoter (BBa_K2070000).
Usage and Biology
![](/wiki/images/d/d5/T--UT-Tokyo--BBa_K2070000--sigma1601.png)
Photograph of plates containing cells transformed with sigma16 related constructs under blue light. From bottom left: BBa_K2070006 (ECF sigma16 inducible GFP reporter), BBa_K2070026 (Constitutive ecf16 generator with ecf 16 inducible GFP reporter), BBa_K2070016 (Constitutive ECF sigma16 generator). As only cells with BBa_K2070026 is shown to fluorescence, it can be suggested that the devices works as expected.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 239
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 434
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |